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DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
https://www.thermofisher.com/order/catalog/product/22582
WebDTNB reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (TNB). The target of DTNB in this reaction is the conjugate base (R—S-) of a free sulfhydryl group. Therefore, the rate of this reaction is dependent on several factors: (1) the reaction pH, (2) the pKa' of the sulfhydryl and (3) steric and ...
DA: 70 PA: 54 MOZ Rank: 41
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Ellman's reagent - Wikipedia
https://en.wikipedia.org/wiki/Ellman%27s_reagent
WebEllman's reagent (5,5′-dithiobis-(2-nitrobenzoic acid) or DTNB) is a colorogenic chemical used to quantify the number or concentration of thiol groups in a sample. It was developed by George L. Ellman. Preparation.
DA: 79 PA: 72 MOZ Rank: 50
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5,5 ?-Dithiobis(2-nitrobenzoic acid) (D8130) - MilliporeSigma
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/707/d8130pis.pdf
WebFrequently called Ellman’s Reagent or DTNB, this was originally developed for use as a sulfhydryl reagent. D8130 is tested for suitability for use as a reagent in sulfhydryl group determination.2 This aromatic disulfide reacts with aliphatic thiol groups to form a mixed disulfide of the protein and one mole of 2-nitro-5-thiobenzoate per mole ...
DA: 1 PA: 40 MOZ Rank: 59
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5,5′-Dithiobis(2-nitrobenzoic acid) - MilliporeSigma
https://www.sigmaaldrich.com/US/en/product/sigma/d8130
Web5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB 2-), to produce 2-nitro-5-thiobenzoate anion (TNB 2-) and one mixed disulfide (R-S-TNB-).TNB 2-has a high …
DA: 45 PA: 95 MOZ Rank: 37
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5,5 -Dithio- bis -(2-nitrobenzoic Acid) - MilliporeSigma
https://www.sigmaaldrich.com/US/en/product/mm/322123
Web5,5′-Dithio-bis-(2-nitrobenzoic Acid), DTNB, Ellman’s Reagent. Empirical Formula (Hill Notation): C 14 H 8 N 2 O 8 S 2. CAS Number: 69-78-3. Molecular Weight: 396.35. MDL number: MFCD00007140. 322123-5GM. $140.00. Availability We apologize but fulfillment and delivery of this product is delayed. We are working to minimize these delays as ...
DA: 4 PA: 79 MOZ Rank: 77
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INSTRUCTIONS Ellman’s Reagent - Thermo Fisher Scientific
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011216_Ellmans_Reag_UG.pdf
WebDTNB reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (TNB; see Figure 2). The target of DTNB in this reaction is the conjugate base (R—S-) of a free sulfhydryl group. Therefore, the rate of this reaction is dependent on several factors: 2
DA: 3 PA: 90 MOZ Rank: 83
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Ellman's Reagent - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/ellmans-reagent
WebEllman's reagent (DTNB) is a colorimetric probe used to quantify the number or concentration of thiol groups in a sample (Ellman, 1959 ). This method is widely disseminated and applied in biological samples to determine biothiols and proteins with free cysteine residues since it is fast, simple, and reproducible, and DTNB is commercially …
DA: 67 PA: 8 MOZ Rank: 65
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Ellman’s assay for Quantification of Sulfhydryl Groups | BMG …
https://www.bmglabtech.com/en/application-notes/ellmans-assay-for-in-solution-quantification-of-sulfhydryl-groups/
WebDTNB reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (TNB; see Figure 2). The target of DTNB in this reaction is the conjugate base (R—S-) of a free sulfhydryl group. TNB is the “colored” species produced in this reaction and has a high molar extinction coefficient in the visible range.
DA: 44 PA: 86 MOZ Rank: 17
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DTNB; Ellman's Reagent, 5,5'-Dithiobis- (2-Nitrobenzoic Acid)
https://www.thermofisher.com/order/catalog/product/D8451
WebDTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
DA: 46 PA: 12 MOZ Rank: 75
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DTNB - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/medicine-and-dentistry/dtnb
WebDTNB (1 mM) is added to both sample and reference. The color develops at 412 nm in about 2–3 min, reaching a plateau that fades slowly. The determination of thiol groups of whole mitochondria requires treatment with a suitable denaturing agent able to expose all thiol groups to the titrating reagent.
DA: 100 PA: 91 MOZ Rank: 30
